diff --git a/apps/browser/src/background/runtime.background.ts b/apps/browser/src/background/runtime.background.ts
index d7aef0db375..9dc2bff65e5 100644
--- a/apps/browser/src/background/runtime.background.ts
+++ b/apps/browser/src/background/runtime.background.ts
@@ -145,6 +145,7 @@ export default class RuntimeBackground {
             if (totpCode != null) {
               this.platformUtilsService.copyToClipboard(totpCode);
             }
+            await this.main.updateOverlayCiphers();
             break;
           }
           case ExtensionCommand.AutofillCard: {
diff --git a/apps/browser/src/vault/popup/services/vault-popup-autofill.service.spec.ts b/apps/browser/src/vault/popup/services/vault-popup-autofill.service.spec.ts
index f271b255c3e..718043b4e85 100644
--- a/apps/browser/src/vault/popup/services/vault-popup-autofill.service.spec.ts
+++ b/apps/browser/src/vault/popup/services/vault-popup-autofill.service.spec.ts
@@ -204,6 +204,7 @@ describe("VaultPopupAutofillService", () => {
 
     describe("doAutofill()", () => {
       it("should return true if autofill is successful", async () => {
+        mockCipher.id = "test-cipher-id";
         mockAutofillService.doAutoFill.mockResolvedValue(null);
         const result = await service.doAutofill(mockCipher);
         expect(result).toBe(true);
@@ -251,6 +252,7 @@ describe("VaultPopupAutofillService", () => {
       });
 
       it("should copy TOTP code to clipboard if available", async () => {
+        mockCipher.id = "test-cipher-id-with-totp";
         const totpCode = "123456";
         mockAutofillService.doAutoFill.mockResolvedValue(totpCode);
         await service.doAutofill(mockCipher);
@@ -405,5 +407,26 @@ describe("VaultPopupAutofillService", () => {
         });
       });
     });
+    describe("handleAutofillSuggestionUsed", () => {
+      const cipherId = "cipher-123";
+
+      beforeEach(() => {
+        mockCipherService.updateLastUsedDate.mockResolvedValue(undefined);
+      });
+
+      it("updates last used date when there is an active user", async () => {
+        await service.handleAutofillSuggestionUsed({ cipherId });
+
+        expect(mockCipherService.updateLastUsedDate).toHaveBeenCalledTimes(1);
+        expect(mockCipherService.updateLastUsedDate).toHaveBeenCalledWith(cipherId, mockUserId);
+      });
+
+      it("does nothing when there is no active user", async () => {
+        accountService.activeAccount$ = of(null);
+        await service.handleAutofillSuggestionUsed({ cipherId });
+
+        expect(mockCipherService.updateLastUsedDate).not.toHaveBeenCalled();
+      });
+    });
   });
 });
diff --git a/apps/browser/src/vault/popup/services/vault-popup-autofill.service.ts b/apps/browser/src/vault/popup/services/vault-popup-autofill.service.ts
index 2d30e857573..3d5b35cded6 100644
--- a/apps/browser/src/vault/popup/services/vault-popup-autofill.service.ts
+++ b/apps/browser/src/vault/popup/services/vault-popup-autofill.service.ts
@@ -16,6 +16,7 @@ import {
 } from "rxjs";
 
 import { AccountService } from "@bitwarden/common/auth/abstractions/account.service";
+import { getOptionalUserId } from "@bitwarden/common/auth/services/account.service";
 import { DomainSettingsService } from "@bitwarden/common/autofill/services/domain-settings.service";
 import { isUrlInList } from "@bitwarden/common/autofill/utils";
 import { I18nService } from "@bitwarden/common/platform/abstractions/i18n.service";
@@ -268,6 +269,7 @@ export class VaultPopupAutofillService {
       });
       return false;
     }
+    await this.handleAutofillSuggestionUsed({ cipherId: cipher.id });
 
     return true;
   }
@@ -326,6 +328,21 @@ export class VaultPopupAutofillService {
     return didAutofill;
   }
 
+  /**
+   * When a user autofills with an autofill suggestion outside of the inline menu,
+   * update the cipher's last used date.
+   *
+   * @param message - The message containing the cipher ID that was used
+   */
+  async handleAutofillSuggestionUsed(message: { cipherId: string }) {
+    const activeUserId = await firstValueFrom(
+      this.accountService.activeAccount$.pipe(getOptionalUserId),
+    );
+    if (activeUserId) {
+      await this.cipherService.updateLastUsedDate(message.cipherId, activeUserId);
+    }
+  }
+
   /**
    * Attempts to autofill the given cipher and, upon successful autofill, saves the URI to the cipher.
    * Will copy any TOTP code to the clipboard if available after successful autofill.